ジャーナル
/
免疫学と感染
/
Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

DOI:
10.3791/51876-v

•

06:05 min

•

September 30, 2014

•

,
1Microbiology and Immunology,University of Michigan, 2Pediatrics and Communicable Diseases,University of Michigan

章

  • 00:05標題
  • 01:07Preparation of the Producer Strain
  • 02:13Overlay Assay
  • 04:25Results: Representative Overlay Outcomes
  • 05:33Conclusion

概要

自動翻訳

自動翻訳

Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species.  Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.

タグ

Overlay AssayBacterial ProductionPneumococcal BacteriocinsStreptococcus PneumoniaeBacteriocin-like Peptide (blp) LocusBacteriocin Immunity Region (BIR)Bacteriocin-mediated CompetitionQuorum Sensing PeptidesLacZ-reporter Strains

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Using Luciferase to Image Bacterial Infections in Mice

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Read Article