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Calculando a média de Viral glicoproteína do envelope Spikes de Electron Cryotomography reconstruções com Jsubtomo
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Calculando a média de Viral glicoproteína do envelope Spikes de Electron Cryotomography reconstruções com Jsubtomo

11,891 Views

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08:29 min

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October 21, 2014

DOI:

10.3791/51714-v

DOI:
10.3791/51714-v

•

08:29 min
October 21, 2014

•

2 Views
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1Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford

概要

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An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.

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Keyword Extraction:Viral Envelope Glycoprotein SpikesElectron CryotomographyJsubtomoViral PathogenesisDrug DesignElectron Cryo-tomographyTomogramsSub-tomogramsBunyamwera VirusBunyaviridae

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Cite this Article

Huiskonen, J. T., Parsy, M., Li, S., Bitto, D., Renner, M., Bowden, T. A. Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo. J. Vis. Exp. (92), e51714, doi:10.3791/51714 (2014).

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