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Haploit Zebra balığı Embriyolar üretimi ile<em> In Vitro</em> Gübreleme
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Production of Haploid Zebrafish Embryos by In Vitro Fertilization

Haploit Zebra balığı Embriyolar üretimi ile<em> In Vitro</em> Gübreleme

DOI:

07:54 min

July 14, 2014

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  • 00:05標題
  • 01:25Preparation
  • 01:53Sample Dissection
  • 03:06UV Sperm Inactivation
  • 03:58In Vitro Fertilization
  • 05:36Observation and Handling of Haploid Embryos
  • 06:09Results: Haploid and Diploid Embryos
  • 07:04Conclusion

概要

自動翻訳

The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.

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