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半数ゼブラフィッシュ胚の生成により<em>体外</em>受精
JoVE Journal
生物学
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JoVE Journal 生物学
Production of Haploid Zebrafish Embryos by In Vitro Fertilization
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半数ゼブラフィッシュ胚の生成により<em>体外</em>受精

24,049 Views

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07:54 min

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July 14, 2014

DOI:

10.3791/51708-v

DOI:
10.3791/51708-v

•

07:54 min
July 14, 2014

•

16 Views
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1Department of Biological Sciences,University of Notre Dame

概要

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The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.

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Cite this Article

Kroeger Jr., P. T., Poureetezadi, S. J., McKee, R., Jou, J., Miceli, R., Wingert, R. A. Production of Haploid Zebrafish Embryos by In Vitro Fertilization. J. Vis. Exp. (89), e51708, doi:10.3791/51708 (2014).

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