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Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
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免疫学と感染
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JoVE Journal 免疫学と感染
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

17,185 Views

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07:31 min

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March 28, 2014

DOI:

10.3791/51345-v

DOI:
10.3791/51345-v

•

07:31 min
March 28, 2014

•

7 Views
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1Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, 2生物学科,University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

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Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.

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Flow CytometryNanoparticle InteractionImmune CellsFITC-SiO2 NanoparticlesRhodamine-SiO2 NanoparticlesPrimary MicrogliaGFP-expressing Monocyte/macrophage LineageCell FluorescenceSide-scattered Light

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Cite this Article

Gamucci, O., Bertero, A., Malvindi, M. A., Sabella, S., Pompa, P. P., Mazzolai, B., Bardi, G. Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry. J. Vis. Exp. (85), e51345, doi:10.3791/51345 (2014).

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