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Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

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07:31 min

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March 28, 2014

DOI:

10.3791/51345-v

DOI:
10.3791/51345-v

•

07:31 min
March 28, 2014

•

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1Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, 2Department of Biology,University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

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Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.

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Flow CytometryNanoparticle InteractionImmune CellsFITC-SiO2 NanoparticlesRhodamine-SiO2 NanoparticlesPrimary MicrogliaGFP-expressing Monocyte/macrophage LineageCell FluorescenceSide-scattered Light

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Cite this Article

Gamucci, O., Bertero, A., Malvindi, M. A., Sabella, S., Pompa, P. P., Mazzolai, B., Bardi, G. Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry. J. Vis. Exp. (85), e51345, doi:10.3791/51345 (2014).

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