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Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
章
- 00:05標題
- 02:55Isolation of Rat Brain Microvessels
- 09:27Purification and Proliferation of RBEC Primocultures
- 10:32Differentiation: Setting up the In Vitro BBB Model
- 14:50Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
- 15:44Conclusion
The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.
