09:27Purification and Proliferation of RBEC Primocultures
10:32Differentiation: Setting up the In Vitro BBB Model
14:50Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.