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Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
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JoVE Journal 免疫学と感染
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

8,665 Views

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11:25 min

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December 26, 2013

DOI:

10.3791/50926-v

DOI:
10.3791/50926-v

•

11:25 min
December 26, 2013

•

3 Views
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1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095,Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique,Hôpital de la Timone

概要

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This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

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Cite this Article

Ouedraogo, R., Daumas, A., Capo, C., Mege, J., Textoris, J. Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation. J. Vis. Exp. (82), e50926, doi:10.3791/50926 (2013).

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