Journal
/
免疫与感染
/
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

DOI:
10.3791/50926-v

•

11:25 min

•

December 26, 2013

•

, , , ,
1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095,Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique,Hôpital de la Timone

Chapters

  • 00:05Title
  • 01:39Preparation of Human CD14+ Monocytes
  • 03:44Differentiation of Monocytes into Macrophages
  • 04:25Preparation of Biological Samples for MALDI-TOF MS
  • 05:08Whole Cell MALDI-TOF MS Analysis
  • 07:22Bioinformatic Analysis
  • 08:05Results: Macrophage Responsiveness
  • 10:56Conclusion

Summary

自动翻译

自动翻译

This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

Tags

MALDI-TOFMass SpectrometryMacrophage ActivationWhole-cell AnalysisProteomic FingerprintingCell IdentificationDiagnostic Applications

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Read Article