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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
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JoVE Journal 神経科学
Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

DOI:
10.3791/50489-v

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09:03 min

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June 13, 2013

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, ,
1Department of Ophthalmology,Northwestern University Feinberg School of Medicine

章

  • 00:05標題
  • 01:34Retinal Preparation
  • 02:22Water Washes and Trypsin Digest
  • 03:33Separating the Vasculature
  • 05:32Flat-mounting the Vasculature
  • 06:13Results: Representative Images of Trypsin Digest
  • 08:07Conclusion

概要

自動翻訳

自動翻訳

Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.

タグ

Retinal VasculatureTrypsin DigestMouse ModelFlat-mount Retinal Blood VesselsRetinal Vascular ChangesMicroaneurysmsCapillary DegenerationEndothelial To Pericyte RatiosMouse EyeRetinal Blood VesselsRetinal Blood Vessel Architecture

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