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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
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June 13, 2013
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Summary
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Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.
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Chou, J. C., Rollins, S. D., Fawzi, A. A. Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model. J. Vis. Exp. (76), e50489, doi:10.3791/50489 (2013).
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