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Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
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免疫学と感染
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JoVE Journal 免疫学と感染
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

22,088 Views

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12:09 min

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February 07, 2013

DOI:

10.3791/50157-v

DOI:
10.3791/50157-v

•

12:09 min
February 07, 2013

•

16 Views
,
1National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch,Centers for Disease Control and Prevention (CDC)

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The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

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Calu-3 CellsAirway Epithelial CellsPolarized CultureLiquid-covered CultureTrans-epithelial Electrical Resistance (TEER)Respiratory PathogensIn Vitro ModelCell CultureAir-liquid Interface

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Harcourt, J. L., Haynes, L. M. Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro. J. Vis. Exp. (72), e50157, doi:10.3791/50157 (2013).

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