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Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

DOI:
10.3791/50157-v

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12:09 min

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February 07, 2013

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1National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch,Centers for Disease Control and Prevention (CDC)

Capitoli

  • 00:05Titolo
  • 01:37Growing Polarized Calu-3 Liquid-covered Cultures (LCC)
  • 04:56Evaluating Resistance Development of Calu-3 LCC
  • 07:43Infecting Polarized Calu-3 LCC with Respiratory Virus
  • 09:47Results: Polarization of Calu-3 LCC and their Response to Respiratory Pathogens
  • 11:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

Tags

Calu-3 CellsAirway Epithelial CellsPolarized CultureLiquid-covered CultureTrans-epithelial Electrical Resistance (TEER)Respiratory PathogensIn Vitro ModelCell CultureAir-liquid Interface

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