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T6SS aracılı Bakteriyel Rekabet Monitör A Görsel Assay
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免疫学と感染
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JoVE Journal 免疫学と感染
A Visual Assay to Monitor T6SS-mediated Bacterial Competition
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T6SS aracılı Bakteriyel Rekabet Monitör A Görsel Assay

15,094 Views

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08:45 min

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March 20, 2013

DOI:

10.3791/50103-v

DOI:
10.3791/50103-v

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08:45 min
March 20, 2013

•

9 Views
, ,
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology,Imperial College London

概要

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Biz aracılık bakteriyel rekabet izlemek için kalitatif bir testtir tarif<em> Pseudomonas aeruginosa</em> Tip VI salgı sistemi (T6SS). Deneyi taşıyan Escherichia coli hedef hücrelerin hayatta kalma / öldürme dayanır<em> LacZ</em>-Muhabiri. Bu teknik T6SS-yetkin mikroorganizmaların bakterisidal / bacteriostasis aktiviteyi değerlendirmek için ayarlanabilir.

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Cite this Article

Hachani, A., Lossi, N. S., Filloux, A. A Visual Assay to Monitor T6SS-mediated Bacterial Competition. J. Vis. Exp. (73), e50103, doi:10.3791/50103 (2013).

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