This content is Free Access.
高通量顺序酶联免疫吸附试验的急性移植物抗宿主病的生物标志物的验证
15,547 Views
•
09:00 min
•
October 31, 2012
DOI:
概要
Automatically generated
多个候选生物标志物的高吞吐量的验证可以通过顺序ELISA进行，以尽量减少冷冻/解冻循环和使用珍贵的血浆样品。在这里，我们演示了如何依次进行验证六种不同的血浆生物标志物的酶联免疫吸附<sup> 1-3</sup的移植物抗宿主病（GVHD）<sup></sup上相同的血浆样品。
Explore More Videos
High Throughput Sequential ELISAValidation Of BiomarkersAcute Graft-Versus-Host DiseaseUnbiased Discovery Proteomics StrategiesNovel BiomarkersDiagnostic TestingPrognostic TestingTherapeutic InterventionsCandidate ProteinsValidate Candidate BiomarkersPatient Plasma SamplesAcute Graft-versus-host DiseaseAllogeneic Hematopoietic Stem Cell TransplantationELISAsGVHD ProteinsIL-2Rα5TNFR16HGF7IL-88Elafin2REG3α3PAP1Sequential ELISA PerformanceFreeze-thaw CyclesThawed Plasma TimePlasma UsagePlasma Biomarker Concentrations
Read Article
Cite this Article
Fiema, B., Harris, A. C., Gomez, A., Pongtornpipat, P., Lamiman, K., Vander Lugt, M. T., Paczesny, S. High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease. J. Vis. Exp. (68), e4247, doi:10.3791/4247 (2012).
Copy