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An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
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January 20, 2013
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The cranial mesenchyme undergoes dramatic morphogenic movements that likely provides a driving force for elevation of the neural folds1,2. Here we describe a simple ex vivo explant assay to characterize the cellular behaviors of the cranial mesenchyme during neurulation. This assay has numerous applications including being amenable to pharmacological manipulations and live imaging analyses.
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Explant AssayCellular BehaviorCranial MesenchymeCentral Nervous SystemNeural PlateNeurulationMorphogenetic MovementsNeural Tube FormationParaxial MesodermNeural Crest CellsPorous MeshworkStellate Shaped CellsExtracellular Matrix (ECM) StrandsStereotypical RearrangementsExpansionDriving ForcePathwaysCellular BehaviorsInteractions Between ECM And CellsEx Vivo AssayPharmacological ManipulationsSignaling PathwaysLive Imaging AnalysesMigratory Properties
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Sarkar, A. A., Zohn, I. E. An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme. J. Vis. Exp. (71), e4245, doi:10.3791/4245 (2013).
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