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小胶质细胞的小分子RNA检测在正常中枢神经系统的实时PCR技术在神经发炎
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免疫学と感染
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JoVE Journal 免疫学と感染
Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
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小胶质细胞的小分子RNA检测在正常中枢神经系统的实时PCR技术在神经发炎

19,227 Views

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13:36 min

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July 23, 2012

DOI:

10.3791/4097-v

DOI:
10.3791/4097-v

•

13:36 min
July 23, 2012

•

9 Views
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1Center for Neurologic Diseases, Brigham and Women’s Hospital,Harvard Medical School

概要

Automatically generated

小胶质细胞的居住提供第一线的防御和免疫监视中枢神经系统的巨噬细胞。 microRNA是调节分子，包括巨噬细胞的激活和分化的许多生理过程中发挥了重要作用。在这篇文章中，我们描述了在小胶质细胞的小分子RNA的测量方法。

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Cite this Article

Veremeyko, T., Starossom, S., Weiner, H. L., Ponomarev, E. D. Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation. J. Vis. Exp. (65), e4097, doi:10.3791/4097 (2012).

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