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A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development
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June 07, 2012
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We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.
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Ray, V. A., Morris, A. R., Visick, K. L. A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development. J. Vis. Exp. (64), e4035, doi:10.3791/4035 (2012).
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