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Chapters
- 00:05Title
- 01:32Initial Characterization and Considerations
- 03:04Spotting Cells onto Solid Agar Media
- 05:36Monitoring Colony Morphology by Microscopy
- 08:14Results: Analysis of Biofilm Development
- 10:49Conclusion
We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.
Tags
Semi-quantitative ApproachAssess Biofilm FormationWrinkled Colony DevelopmentBacteriaExtracellular MatrixPhysiologyResistance To AntibioticsEnvironmental StressesHost-microbe InteractionsPlanktonic CellsComplex CommunitiesLaboratory StudyBiofilm PhenotypesWrinkled Or Rugose Bacterial ColoniesSolid Agar MediaIndicator Of Biofilm FormationGram-positive BacteriaGram-negative BacteriaBacillus SubtilisVibrio CholeraeVibrio ParahaemolyticusPseudomonas AeruginosaVibrio Fischeri
