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Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
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May 08, 2012
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This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.
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Low-intensity UltrasoundAdhesion-dependent SignalsMulticellular OrganismsCell BehaviorExtracellular MatrixMatrix-engagementCell MigrationCell ProliferationCell SecretionCell DifferentiationMolecular InteractionsExtracellular Matrix ReceptorsIntegrinsMechanical LinkAdhesion-dependent Signaling CascadesTransmembrane ReceptorsSyndecan-4α5β1-integrinFibronectinSyndecan-4 SignalingFibroblast MigrationHealing RetardationFibronectin-dependent SignalingRepair Processes
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Roper, J., Harrison, A., Bass, M. D. Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound. J. Vis. Exp. (63), e4024, doi:10.3791/4024 (2012).
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