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Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
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January 07, 2012
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We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.
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Intravital ImagingMouse Thymus2-photon MicroscopyImage AcquisitionOrgansStereotactic ToolsSurgical ProceduresCellular BehaviorsLive AnimalsEx Vivo Imaging AcquisitionSurgical ProcedureOrgan HolderFoxp3+ CellsRegulatory T CellsNaturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo InteractionsPhenotype And PhysiologyNude Mice
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Caetano, S. S., Teixeira, T., Tadokoro, C. E. Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy. J. Vis. Exp. (59), e3504, doi:10.3791/3504 (2012).
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