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Fluorescence detection methods for microfluidic droplet platforms
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生物工学
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JoVE Journal 生物工学
Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

DOI:
10.3791/3437-v

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14:16 min

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December 10, 2011

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, , , , , ,
1化学科,Imperial College London , 2Department of Biochemistry, Protein Chip Research Center,Chungbuk National University, 3Department of Chemistry and Applied Biosciences,Institute for Chemical and Bioengineering, ETH Zurich

章

  • 00:05標題
  • 00:27Introduction
  • 02:12Microchip Fabrication
  • 04:07Microfluidic Setup
  • 05:55Optical Setup
  • 08:00Data Acquisition
  • 09:00Cell Recognition
  • 11:13Mapping of Mixing Events Using FLIM
  • 13:04Conclusion

概要

自動翻訳

自動翻訳

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

タグ

Fluorescence Detection MethodsMicrofluidic Droplet PlatformsSystem MiniaturizationNano-to-femto-liter VolumesFunctional Components IntegrationLarge-scale MultiplexingAnalytical ThroughputInstrumental Footprints ReductionDroplet-based Microfluidic SystemsHigh-throughput ExperimentationWater-in-oil EmulsionsCapillary InstabilitiesMicrodroplets GenerationIsolated CompartmentsSample Cross-talk EliminationDispersion ReductionMinimal Cross-contaminationAccurate Timing Of Analytical ProcessesContact-free Environment

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