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Fluorescence detection methods for microfluidic droplet platforms
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December 10, 2011
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Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.
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Fluorescence Detection MethodsMicrofluidic Droplet PlatformsSystem MiniaturizationNano-to-femto-liter VolumesFunctional Components IntegrationLarge-scale MultiplexingAnalytical ThroughputInstrumental Footprints ReductionDroplet-based Microfluidic SystemsHigh-throughput ExperimentationWater-in-oil EmulsionsCapillary InstabilitiesMicrodroplets GenerationIsolated CompartmentsSample Cross-talk EliminationDispersion ReductionMinimal Cross-contaminationAccurate Timing Of Analytical ProcessesContact-free Environment
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Casadevall i Solvas, X., Niu, X., Leeper, K., Cho, S., Chang, S., Edel, J. B., deMello, A. J. Fluorescence detection methods for microfluidic droplet platforms. J. Vis. Exp. (58), e3437, doi:10.3791/3437 (2011).
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