このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
10,688 Views
•
11:26 min
•
December 06, 2011
DOI:
概要
Automatically generated
The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.
Explore More Videos
ImagingNeuronal ResponsesSlice PreparationsVomeronasal OrganGenetically Encoded Calcium SensorChemosensory SignalsSocialSexualReproductive StatusSpeciesPheromonesPredatorsVomeronasal Sensory NeuronsSpecificitySensitivityG-protein Coupled ReceptorsV1RV2RFPRDetection Of Chemosensory CuesPheromone Information EncodingResponse ProfilesStimuliSpecific ReceptorsNeuroepithelia Of VNOVomer BonesSemi-blind Tubular StructureVomeronasal DuctNasal CavityDendritesLumen Part Of VNOPheromone Cues Contact With ReceptorsCell Bodies Of VSNs Form Pseudo-stratified Layers
Read Article
Cite this Article
Ma, L., Haga-Yamanaka, S., Yu, Q. E., Qiu, Q., Kim, S., Yu, C. R. Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor. J. Vis. Exp. (58), e3404, doi:10.3791/3404 (2011).
Copy