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Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
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Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
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11:26 min
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December 06, 2011
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Summary
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The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.
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ImagingNeuronal ResponsesSlice PreparationsVomeronasal OrganGenetically Encoded Calcium SensorChemosensory SignalsSocialSexualReproductive StatusSpeciesPheromonesPredatorsVomeronasal Sensory NeuronsSpecificitySensitivityG-protein Coupled ReceptorsV1RV2RFPRDetection Of Chemosensory CuesPheromone Information EncodingResponse ProfilesStimuliSpecific ReceptorsNeuroepithelia Of VNOVomer BonesSemi-blind Tubular StructureVomeronasal DuctNasal CavityDendritesLumen Part Of VNOPheromone Cues Contact With ReceptorsCell Bodies Of VSNs Form Pseudo-stratified Layers
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Ma, L., Haga-Yamanaka, S., Yu, Q. E., Qiu, Q., Kim, S., Yu, C. R. Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor. J. Vis. Exp. (58), e3404, doi:10.3791/3404 (2011).
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