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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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December 08, 2011
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Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.
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Vaginal InoculationSample CollectionExperimental Mouse ModelCandida VaginitisVulvovaginal CandidiasisCandida SpeciesFungal InfectionLower Female Genital TractPredisposing FactorsAntibiotic UsageUncontrolled DiabetesReproductive Hormone LevelsPregnancyOral ContraceptivesHormone Replacement TherapiesRecurrent VVCExperimental Mouse Model Of VVCPathogenesisMucosal Host ResponseAntifungal TherapiesPseudoestrusCandida Colonization/infection
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Yano, J., Fidel, Jr., P. L. Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis. J. Vis. Exp. (58), e3382, doi:10.3791/3382 (2011).
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