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Following Cell-fate in E. coli After Infection by Phage Lambda
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October 14, 2011
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This article describes the procedure for preparing a fluorescently-labeled version of bacteriophage lambda, infection of E. coli bacteria, following the infection outcome under the microscope, and analysis of infection results.
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Cell-fateE. ColiInfectionPhage LambdaLytic PathwayLysogenic PathwayFluorescent LabelingReal-time MicroscopyIndividual PhagesIndividual CellsInfection ExperimentsFluorescent PhagesCapsid GpD ProteinYFP-fusionCrude Phage LysatePurification StepsDAPI-labelingMicroscope ImagingAdsorptionViral DNA Injection
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Zeng, L., Golding, I. Following Cell-fate in E. coli After Infection by Phage Lambda. J. Vis. Exp. (56), e3363, doi:10.3791/3363 (2011).
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