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Following Cell-fate in E. coli After Infection by Phage Lambda
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

DOI:
10.3791/3363-v

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06:10 min

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October 14, 2011

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1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 00:58Creation of a Crude Phage Lysate
  • 01:56Preparation of Agarose Gel Slabs
  • 02:33Phage Infection
  • 03:27Following Cell Fate under the Microscope
  • 04:42Cycle of Phage Infection
  • 05:45Conclusion

Summary

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This article describes the procedure for preparing a fluorescently-labeled version of bacteriophage lambda, infection of E. coli bacteria, following the infection outcome under the microscope, and analysis of infection results.

Tags

Cell-fateE. ColiInfectionPhage LambdaLytic PathwayLysogenic PathwayFluorescent LabelingReal-time MicroscopyIndividual PhagesIndividual CellsInfection ExperimentsFluorescent PhagesCapsid GpD ProteinYFP-fusionCrude Phage LysatePurification StepsDAPI-labelingMicroscope ImagingAdsorptionViral DNA Injection

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