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特派团LentiPlex汇集在哺乳动物细胞中的shRNA文库筛选
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

特派团LentiPlex汇集在哺乳动物细胞中的shRNA文库筛选

DOI:

09:12 min

December 21, 2011

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  • 00:05標題
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

概要

自動翻訳

在这里,我们使用人类LentiPlex汇集库和传统的测序方法,以确定靶基因,促进细胞的存活。我们演示了如何设置和deconvolute一个LentiPlex屏幕和验证的结果。

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