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特派团LentiPlex汇集在哺乳动物细胞中的shRNA文库筛选
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09:12 min
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December 21, 2011
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概要
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在这里，我们使用人类LentiPlex汇集库和传统的测序方法，以确定靶基因，促进细胞的存活。我们演示了如何设置和deconvolute一个LentiPlex屏幕和验证的结果。
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MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes
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Cite this Article
Coussens, M. J., Corman, C., Fischer, A. L., Sago, J., Swarthout, J. MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells. J. Vis. Exp. (58), e3305, doi:10.3791/3305 (2011).
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