Journal
/
Biologie
/
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

DOI:
10.3791/3305-v

•

09:12 min

•

December 21, 2011

•

, , , ,
1Sigma-Aldrich

Kapitel

  • 00:05Titel
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.

Tags

MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Trypsinizing and Subculturing Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Analysis of Cell Cycle Position in Mammalian Cells

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Measurement of Heme Synthesis Levels in Mammalian Cells

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Read Article