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Avaliação do Câncer de Migração Stem Cell Usando compartimentar dispositivos microfluídicos e imagens de células vivas
1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
章
- 00:05標題
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
Um dispositivo de microfluidos para a investigação de compartimentalizar cancro migração de células estaminais é descrito. Esta plataforma inovadora cria um microambiente viável celular e permite a visualização microscópica de locomoção de células vivas. Altamente móveis células cancerosas são isolados para estudar os mecanismos moleculares de infiltração agressiva, levando potencialmente a terapias mais eficazes no futuro.
タグ
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell Imagingがん研究Cellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
