ジャーナル
/
生物工学
/
Preparação de amostras, Imaging, and Analysis Protocolos para Faca de ponta microscopia de varredura
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Preparação de amostras, Imaging, and Analysis Protocolos para Faca de ponta microscopia de varredura

17,431 Views

•

10:25 min

•

December 09, 2011

DOI:

10.3791/3248-v

DOI:
10.3791/3248-v

•

10:25 min
December 09, 2011

•

2 Views
, , , , , , , ,
1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3電気・コンピュータ工学科,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University

概要

Automatically generated

Todo o processo de preparação de amostras de imagens do cérebro para o seccionamento serial usando o microscópio de varredura Knife Edge, para visualização e análise de dados é descrito. Esta técnica é usada atualmente para adquirir dados cérebro do rato, mas é aplicável a outros órgãos, outras espécies.

Explore More Videos

Specimen PreparationImagingAnalysis ProtocolsKnife-edge Scanning MicroscopyHigh-throughput MicroscopyHigh-resolution Microscopy3D MicroscopyNeuroanatomical DataSubmicrometer ResolutionWhole-brain-scale DataNeuronal NetworksVascular NetworksCell Body DistributionConnectomics ResearchMicrocirculation ResearchHemodynamic ResearchStereology ResearchFixingStainingEmbeddingKESM Configuration And SetupSectioning And Imaging With KESMImage ProcessingData PreparationData VisualizationData Analysis

Read Article

Cite this Article

Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., Abbott, L. C. Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy. J. Vis. Exp. (58), e3248, doi:10.3791/3248 (2011).

Download .ris file

Copy

Share Video

.

Copy