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Probenvorbereitung, Imaging-und Analyse-Protokolle für Knife-edge-Scanning-Mikroskopie
JoVE Journal
生物工学
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JoVE Journal 生物工学
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
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Probenvorbereitung, Imaging-und Analyse-Protokolle für Knife-edge-Scanning-Mikroskopie

17,431 Views

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10:25 min

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December 09, 2011

DOI:

10.3791/3248-v

DOI:
10.3791/3248-v

•

10:25 min
December 09, 2011

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2 Views
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1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3電気・コンピュータ工学科,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University

概要

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Der gesamte Prozess vom Gehirn Probenvorbereitung auf serielle Schnitte Bildgebung mit dem Knife-Edge-Scanning-Mikroskop, um Daten-Visualisierung und Analyse beschrieben. Diese Technik wird derzeit verwendet, um Maus-Hirn-Daten zu erfassen, aber es ist auf andere Organe, andere Arten.

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Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., Abbott, L. C. Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy. J. Vis. Exp. (58), e3248, doi:10.3791/3248 (2011).

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