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Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
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September 15, 2011
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An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.
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Lectin-based IsolationCultureMouse Embryonic MotoneuronsSpinal MotoneuronsNeuroepithelial CellsNkx6.1Precursor CellsPostmitotic StagesDendritesAxonsTranscription FactorsIslet 1/2Innervate MusclesHigh Affinity ReceptorsBrain Derived Neurotrophic Factor (BDNF)Neurotrophin-3 (NT-3)Tropomyosin-related Kinase B And C (TrkBTrkC)Tropomyosin-related Kinase A (TrkA)Low Affinity Neurotrophin Receptor P75NTRIsolation TechniquesSpecific Antibodies (panning)
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Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S., Klausmeyer, A. Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons. J. Vis. Exp. (55), e3200, doi:10.3791/3200 (2011).
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