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Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
Chapters
- 00:05Title
- 01:37PORN-H/Laminin Coating of Dishes and Coverslips
- 02:33Lectin Coating of the Purification Plate
- 03:20Motoneuron Culture Medium
- 04:32Spinal Cord Dissection
- 05:35Enrichment of Motoneurons by Lectin-based Purification
- 07:28Results: Lectin-based Isolation and Characterization of Mouse Embryonic Motoneurons
- 08:46Conclusion
An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.
Tags
Lectin-based IsolationCultureMouse Embryonic MotoneuronsSpinal MotoneuronsNeuroepithelial CellsNkx6.1Precursor CellsPostmitotic StagesDendritesAxonsTranscription FactorsIslet 1/2Innervate MusclesHigh Affinity ReceptorsBrain Derived Neurotrophic Factor (BDNF)Neurotrophin-3 (NT-3)Tropomyosin-related Kinase B And C (TrkBTrkC)Tropomyosin-related Kinase A (TrkA)Low Affinity Neurotrophin Receptor P75NTRIsolation TechniquesSpecific Antibodies (panning)
