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1Institute for Memory Impairments and Neurological Disorders,University of California, 2Physical Medicine & Rehabilitation,University of California, 3Anatomy & Neurobiology,University of California, 4Sue and Bill Gross Stem Cell Research Center,University of California, 5Section of Molecular Biology,University of California, 6Reeve-Irvine Research Center,University of California
章
- 00:05標題
- 01:23Dissociation of Spinal Cord Tissue
- 02:47Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
- 04:27Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
- 06:16Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
- 07:33Conclusion
Kwantificering van cellulaire ontsteking in de benadeelde / pathologische CNS door flow cytometrie wordt bemoeilijkt door lipiden / myeline puin dat vergelijkbare grootte en granulatie, om cellen te kunnen hebben, het verminderen van gevoeligheid / nauwkeurigheid. We hebben een geavanceerde celpreparaat methode om myeline vuil te verwijderen en te verbeteren cel detectie door middel van flowcytometrie in de geblesseerde ruggenmerg.
タグ
Quantitative AssessmentImmune CellsFlow CytometryCell Purification MethodCentral Nervous System (CNS)Cellular InflammationMorphological TechniquesHistological TechniquesAlternative MethodMyelin DebrisOptiPrep Gradient SystemSensitive QuantificationsReliable QuantificationsInjury SeverityCharacterization
