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Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
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Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
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April 09, 2011
DOI:
1Institute for Memory Impairments and Neurological Disorders,University of California, 2Physical Medicine & Rehabilitation,University of California, 3Anatomy & Neurobiology,University of California, 4Sue and Bill Gross Stem Cell Research Center,University of California, 5Section of Molecular Biology,University of California, 6Reeve-Irvine Research Center,University of California
Summary
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Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.
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Quantitative AssessmentImmune CellsFlow CytometryCell Purification MethodCentral Nervous System (CNS)Cellular InflammationMorphological TechniquesHistological TechniquesAlternative MethodMyelin DebrisOptiPrep Gradient SystemSensitive QuantificationsReliable QuantificationsInjury SeverityCharacterization
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Nguyen, H. X., Beck, K. D., Anderson, A. J. Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method. J. Vis. Exp. (50), e2698, doi:10.3791/2698 (2011).
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