The chicken auditory brainstem is comprised of nuclei responsible for binaural sound processing. A single coronal slice preparation maintains the entire circuitry while the cultured approach provides a unique preparation to study the development of neuronal structure and auditory function at the molecular, cellular and network levels.
Sanchez, J. T., Seidl, A. H., Rubel, E. W., Barria, A. Preparation and Culture of Chicken Auditory Brainstem Slices. J. Vis. Exp. (49), e2527, doi:10.3791/2527 (2011).