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Preparation and Culture of Chicken Auditory Brainstem Slices
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Journal JoVE Neurosciences
Preparation and Culture of Chicken Auditory Brainstem Slices

Preparation and Culture of Chicken Auditory Brainstem Slices

10,909 Views

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11:16 min

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March 21, 2011

DOI:

10.3791/2527-v

DOI:
10.3791/2527-v

•

11:16 min
March 21, 2011

•

3 Views
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1Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center,University of Washington, 2Department of Physiology and Biophysics, Virginia Merrill Bloedel Hearing Research Center,University of Washington

Summary

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The chicken auditory brainstem is comprised of nuclei responsible for binaural sound processing. A single coronal slice preparation maintains the entire circuitry while the cultured approach provides a unique preparation to study the development of neuronal structure and auditory function at the molecular, cellular and network levels.

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Cite this Article

Sanchez, J. T., Seidl, A. H., Rubel, E. W., Barria, A. Preparation and Culture of Chicken Auditory Brainstem Slices. J. Vis. Exp. (49), e2527, doi:10.3791/2527 (2011).

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