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Estabelecer embrionárias de rato Cultura de Células-tronco neurais utilizando o ensaio Neurosphere
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JoVE Journal 神経科学
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Estabelecer embrionárias de rato Cultura de Células-tronco neurais utilizando o ensaio Neurosphere

24,383 Views

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11:54 min

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January 11, 2011

DOI:

10.3791/2457-v

DOI:
10.3791/2457-v

•

11:54 min
January 11, 2011

•

22 Views
, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

概要

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Este protocolo de vídeo demonstra a aplicação do ensaio neurosphere para o isolamento e expansão de células-tronco neurais do eminências ganglionares do dia 14 de embriões de rato-cérebro.

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Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).

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