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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
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January 05, 2011
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概要
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This protocol is a cost effective alternative for efficient parallel clarification and plasmid DNA purification from E. coli cultures. The AcroPrep Advance process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate.
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Plasmid DNA PurificationAcroPrep Advance Filter PlatesProkaryotic CulturesBacterial Lysate ClearanceDNA PurificationStreamlined ProcessSilica-based MediaVacuum FiltrationCentrifugeHigh-quality DNAOD260/280 RatiosDownstream ApplicationsSequencingCloningManual ProcessingSemi-automated ProcessingFully-automated Processing
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Pueschel, L., Li, H., Hymes, M. Streamlined Purification of Plasmid DNA From Prokaryotic Cultures. J. Vis. Exp. (47), e2407, doi:10.3791/2407 (2011).
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