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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
DOI:

06:53 min

January 05, 2011

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Chapters

  • 00:05Title
  • 01:02Lysate Clearance
  • 02:58DNA Binding
  • 04:10DNA Recovery
  • 05:16Quantitative and Qualitative Analyses
  • 06:30Conclusion

Summary

自动翻译

This protocol is a cost effective alternative for efficient parallel clarification and plasmid DNA purification from E. coli cultures. The AcroPrep Advance process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate.

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