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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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JoVE Journal 免疫学と感染
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

DOI:
10.3791/2287-v

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07:19 min

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October 21, 2010

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, , ,
1生物学科,University of Texas San Antonio – UTSA, 2South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio – UTSA

章

  • 00:00標題
  • 01:24Preparation of C. albicans
  • 02:49Setting Up the 96-well Microtiter Plate for the Formation of the Biofilm
  • 03:55Antifungal Susceptibility Testing of Biofilms
  • 06:02Evaluation of Antifungal Reagents
  • 06:43Conclusion

概要

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自動翻訳

We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

タグ

96 Well Microtiter PlateMonitoringFormationAntifungal Susceptibility TestingCandida AlbicansBiofilmsCandidiasisFungal InfectionsMedical DevicesCathetersIn Vitro ModelColorimetric AssayXTTTetrazolium SaltMetabolically Active Fungal Biofilm CellsExperiment DurationAntifungal Susceptibility Testing Standardization

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