Rivista
/
Immunologia e infezioni
/
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

DOI:
10.3791/2287-v

•

07:19 min

•

October 21, 2010

•

, , ,
1Department of Biology,University of Texas San Antonio – UTSA, 2South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio – UTSA

Capitoli

  • 00:00Titolo
  • 01:24Preparation of C. albicans
  • 02:49Setting Up the 96-well Microtiter Plate for the Formation of the Biofilm
  • 03:55Antifungal Susceptibility Testing of Biofilms
  • 06:02Evaluation of Antifungal Reagents
  • 06:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

Tags

96 Well Microtiter PlateMonitoringFormationAntifungal Susceptibility TestingCandida AlbicansBiofilmsCandidiasisFungal InfectionsMedical DevicesCathetersIn Vitro ModelColorimetric AssayXTTTetrazolium SaltMetabolically Active Fungal Biofilm CellsExperiment DurationAntifungal Susceptibility Testing Standardization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Microtiter Dish Biofilm Formation Assay

In vitro Biofilm Formation in an 8-well Chamber Slide

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

Clarifying and Imaging Candida albicans Biofilms

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans

Read Article