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An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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JoVE Journal 免疫学と感染
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

DOI:
10.3791/2146-v

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10:35 min

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November 28, 2010

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, ,
1Penn Ovarian Cancer Research Center, Center for Research on Reproduction and Womans Health, Department of Obstetrics and Gynecology,University of Pennsylvania-School of Medicine, 2Women’s Cancer Program,Fox Chase Cancer Center

章

  • 00:05標題
  • 01:08Introduction
  • 01:45The Syngeneic Model
  • 03:43Cell Culture
  • 04:34Pre-Surgery
  • 05:48Surgery
  • 07:27In vivo Imaging
  • 09:20Representative in vivo and ex vivo Images
  • 10:06Conclusion

概要

自動翻訳

自動翻訳

To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.

タグ

Orthotopic ModelSerous Ovarian CancerImmunocompetent MiceIn Vivo Tumor ImagingTumor Immune ResponsesTg-MISIIR-TAg Transgenic Mouse ModelSV40 Transforming RegionMullerian Inhibitory Substance Type II Receptor (MISIIR) Gene PromoterSyngeneic Allograft RecipientsPreclinical StudiesTumor Microenvironment

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