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Simplified and Efficient AAV Production Using HEK293 Suspension Culture
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Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production
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神経科学
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JoVE Journal 神経科学
Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

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03:36 min

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April 26, 2024

DOI:

10.3791/201531-v

DOI:
10.3791/201531-v

•

03:36 min
April 26, 2024

•

2 Views
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1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

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Pietersz, K. L., Nijhuis, P. J., Klunder, M. H., van den Herik, J., Hobo, B., de Winter, F., Verhaagen, J. Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production. J. Vis. Exp. (Pending Publication), e201531, doi:10.3791/201531 (2024).

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