Journal
/
神经科学
/
Simplified and Efficient AAV Production Using HEK293 Suspension Culture
/
Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

86 Views

•

03:36 min

•

April 26, 2024

DOI:

10.3791/201531-v

DOI:
10.3791/201531-v

•

03:36 min
April 26, 2024

•

2 Views
, , , , , ,
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Explore More Videos

Iodixanol GradientHEK293 Cell LysatesAdeno-associated Virus ProductionPEG PrecipitationDNase I TreatmentUltracentrifugationVirus ExtractionCentrifugal FiltrationPBSSucrose

Read Article

Cite this Article

Pietersz, K. L., Nijhuis, P. J., Klunder, M. H., van den Herik, J., Hobo, B., de Winter, F., Verhaagen, J. Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production. J. Vis. Exp. (Pending Publication), e201531, doi:10.3791/201531 (2024).

Download .ris file

Copy

Share Video

.

Copy