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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
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生物工学
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JoVE Journal 生物工学
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

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03:44 min

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May 19, 2023

DOI:

10.3791/200259-v

DOI:
10.3791/200259-v

•

03:44 min
May 19, 2023

•

1 Views
, , ,
1Department of Obstetrics,University Hospital Zurich, University of Zurich, 2Ectica Technologies AG

タグ

3D Stromal-endothelial CellsOsteogenic DifferentiationAlkaline Phosphatase ActivityHBM-MSCsGFP-HUVECsBCIP/NBT SubstrateCell LysatesALP Activity QuantificationDNA QuantificationMesenchymal Stem Cells

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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity. J. Vis. Exp. (Pending Publication), e200259, doi:10.3791/200259 (2023).

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